Effects of oxysophoridine on amino acids after cerebral ischemic injury in mice
Tengfei Wang1, Yuxiang Li2, Peng Zhao1, Jie Wang3, Xiaomin Zhang3, Yinju Hao1, Juan Du1, Chengjun Zhao4, Tao Sun5, Jianqiang Yu6, Ru Zhou1, Shaoju Jin1
1 Department of Pharmacology, Ningxia Medical University, Yinchuan 750004, China
2 College of Nursing, Ningxia Medical University, Yinchuan 750004, China
3 Technology Centre, Ningxia Medical University, Yinchuan 750004, China
4 Key Laboratory of Fertility Preservation and Maintenance, Ningxia Medical University, Yinchuan 750004, China
5 Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan 750004, China
6 Department of Pharmacology; Ningxia Hui Medicine Modern Engineering Research Center, Ningxia Medical University, Yinchuan 750004, China
Department of Pharmacology, Ningxia Medical University,1160 Shengli Street, Yinchuan, 750004,Ningxia
Source of Support: 2012 “Western light” talent cultivation plan in Chinese Academy of Sciences, the Key Scientific Research Project of Ningxia health department (grant no.
2012152) and the Research Project of Ningxia education department (grant no. NGY2012055)., Conflict of Interest: None
Background: Our previous studies demonstrated that oxysophoridine (OSR) had neuroprotective effects on mice through antioxidant and anti-apoptotic mechanisms. In this study, we investigated whether OSR could influence the release of amino acids in ischemic mice brains. Materials and Methods: Male ICR mice were scheduled to undergo 2 h middle cerebral artery occlusion (MCAO) and 24 h reperfusion. Before MCAO, mice in corresponding groups were intraperitoneally injected with OSR (62.5, 125 and 250 mg/kg) for seven successive days. After reperfusion, neurological scores were estimated, infarct volume and the brain water content were assessed. The levels of glutamate (Glu), aspartate (Asp), γ-aminobutyric acid (GABA) and Glycine (Gly) were measured by amino acid analyzer. Results: OSR significantly decreased neurological scores, reduced infarct volume and the brain water content. After treatment with OSR of 250 mg/kg, the contents of Glu, Asp, GABA and Gly in mice brains could maintain at a normal level compared with MCAO group mice. The Glu/GABA ratio was significantly decreased in OSR group mice. Conclusion: These findings indicate that OSR has a protective effect on cerebral ischemic injury and helps to maintain the amino acids homeostasis after reperfusion for a long time.