Annals of Indian Academy of Neurology
  Users Online: 2026 Home | About the Journal | InstructionsCurrent Issue | Back IssuesLogin      Print this page Email this page  Small font size Default font size Increase font size
Year : 2018  |  Volume : 21  |  Issue : 4  |  Page : 250-255

Restriction fragment length polymorphism-based genotyping of Toxoplasma gondii from autopsy-proven cases of acquired immunodeficiency syndrome-associated cerebral toxoplasmosis

1 Department of Microbiology, Kidwai Cancer Institute, Bengaluru, Karnataka, India
2 Defence Food Research Laboratory, Defence Research and Development Organisation, Siddhartha Nagar, Mysore, Karnataka, India
3 Department of Pathology, National Institute of Mental Health and Neuro Sciences, Bengaluru, Karnataka, India

Correspondence Address:
Prof. R S Jayshree
Department of Microbiology, Kidwai Cancer Institute, Hosur Road, Bengaluru - 560 029, Karnataka
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/aian.AIAN_358_17

Rights and Permissions

Context: Published data on genetic characterization of Toxoplasma gondii (T.gondii) from clinical cases of toxoplasmosis from India is lacking. Aims: The present study was aimed at identifying genetic types of T. gondii in fatal cases of cerebral toxoplasmosis (CT) associated with HIV, from India. Settings and Design: Archived tissues of CT were obtained postmortem from 25 acquired immunodeficiency syndrome patients between 2000 and 2014. Subjects and Methods: Direct amplification of eight different loci, namely, SAG1, 5'-3'SAG2, Alt. SAG2, SAG3, BTUB, GRA6, C22-8, and L358 followed by restriction fragment length polymorphism was used to genotype the parasite. Results: The canonical Types I, II, or III were not found in our study. More than 96% of the cases harbored atypical genotypes–likely recombinants of the canonical types; one case closely corresponded to Type II genotype. Conclusions: Thus, a majority of T. gondii causing CT in South India belonged to a noncanonical lineage. These nonarchetypal genotypes differed from the conventional Types I, II, and III and caused devastating severity in patients with CT in the background of HIV. These results are a step further to deciphering the population genetics of this important zoonotic parasitic infection in Indian patients, information that has thus far been lacking.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded66    
    Comments [Add]    

Recommend this journal